I. Definition and Overview

An Integrated Staining and Sealing Fume Hood is a specialized laboratory ventilation system designed to combine the processes of staining (染色, staining treatment of materials or biological samples) and sealing (封闭, protective or containment sealing) within a single enclosed structure. It ensures a controlled environment by providing local exhaust ventilation (LEV), preventing hazardous fumes, vapors, or particulates from escaping into the laboratory.

Key terminology:

• HEPA (High-Efficiency Particulate Air) Filter – capable of removing ≥99.97% of particles ≥0.3 μm.
• ULPA (Ultra-Low Penetration Air) Filter – efficiency ≥99.999% for 0.12 μm particles.
• Airflow Velocity – the linear speed of air across the work area, typically 0.3–0.5 m/s.
• ISO 14644-1: Cleanrooms and Associated Controlled Environments – the international standard for cleanroom classification.

II. Working Principle

The fume hood operates based on a controlled airflow circulation system:

• Air Inflow: Ambient air enters the hood through the front opening to create a negative pressure barrier.
• Filtration System: Exhaust air passes through multi-stage filters (pre-filter + HEPA/ULPA) to remove particulates and contaminants.
• Exhaust and Recirculation: Cleaned air is either discharged outside or recirculated depending on system design.
• Cross-Contamination Prevention: Segregated air channels ensure that staining and sealing processes remain isolated.
• Automatic Control System: Equipped with sensors, airflow monitors, and alarms to comply with GMP (Good Manufacturing Practice) and ISO standards.

III. Main Functions

• Minimizes cross-contamination risk between processes.
• Maintains a sterile and clean working environment.
• Protects operators from chemical exposure and harmful vapors.
• Enhances productivity by integrating two processes into one system.
• Ensures compliance with ISO 14644-1 and FDA cleanroom requirements.

IV. Structural Features and Materials

The integrated fume hood is manufactured using durable and easy-to-clean materials:

• Stainless steel (SS304/SS316) – corrosion-resistant, hygienic.
• Powder-coated cold-rolled steel – robust structural support.
• Aluminum alloy frames – lightweight yet strong.
• Tempered glass – chemical resistant and transparent viewing.
• Seamless arc-shaped corners – prevent dust accumulation and allow easy cleaning.

V. Classification and Technical Parameters

VI. Typical Application Industries

The integrated staining and sealing fume hood is widely applied in:

• Cleanroom Engineering
• Medical Engineering
• Construction & Architectural Engineering
• Laboratory Engineering
• Medical Devices & Equipment Manufacturing
• Pharmaceutical and Biotech Facilities
• Environmental Protection & Green Technology

VII. Installation and Usage Considerations

• Must comply with GMP standards and ISO 14644-1 cleanroom requirements.
• Install in areas with stable airflow, avoiding direct drafts from doors or HVAC vents.
• Ensure proper electrical grounding and compliance with safety codes.
• Routine calibration of airflow velocity monitors is recommended.

VIII. Maintenance and Care

• Regularly clean and disinfect all interior surfaces.
• Replace pre-filters every 3–6 months; HEPA/ULPA filters every 12–18 months (depending on usage).
• Check and recalibrate control systems and airflow monitors.
• Lubricate mechanical parts and inspect seals to ensure airtightness.
• Maintain detailed service logs for regulatory compliance.

IX. Advantages

• Integration of staining and sealing reduces equipment footprint.
• Improved operator safety through advanced containment systems.
• Energy-efficient design lowers operational costs.
• Modular structure allows customization for different industries.
• Long service life with minimal maintenance requirements.